gnf351 calbiochem Search Results


gnf 351 calbiochem  (MedChemExpress)
93
MedChemExpress gnf 351 calbiochem
Gnf 351 Calbiochem, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gnf 351 calbiochem/product/MedChemExpress
Average 93 stars, based on 1 article reviews
gnf 351 calbiochem - by Bioz Stars, 2026-02
93/100 stars
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gnf-351  (Millipore)
90
Millipore gnf-351
AhR antagonists inhibit AhR activation by butyrate. HT-29-AhR reporter cells ( A ) and Caco-2-AhR reporter cells ( B ) were incubated with AhR ligand antagonists (CH-223191 1 µM and <t>GNF-351</t> 1 µM) and an HSP90 inhibitor ((−)-Epigallocatechin-3-gallate, EGCG, 20 µM) prior stimulation with butyrate (But, 2 mM) and TCDD (10 nM) for 24 h. ( C ) Relative expression of CYP1A1 on HT-29 cells treated during 6 h with 2 mM butyrate or TCDD 10 nM in presence or absence of AhR antagonists. CYP1A1 expression is normalised to control expression (unstimulated cells) and is determined by the 2 −ΔΔCt method using β-actin as control gene. ( D ) Relative expression of CYP1A1 on HT-29 cells transfected with control siRNA (white bars) or AhR siRNA (grey bars) and incubated during 6 h with 2 mM butyrate or TCDD 10 nM. CYP1A1 expression is normalised to stimulated control expression (cells transfected with control siRNA and stimulated with either butyrate or TCDD), expressed in percentage and is determined by the 2 −ΔΔCt method using β-actin as control gene. Data are means ± SEM of at least three distinct experiments, performed in triplicate. ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001.
Gnf 351, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gnf-351/product/Millipore
Average 90 stars, based on 1 article reviews
gnf-351 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


AhR antagonists inhibit AhR activation by butyrate. HT-29-AhR reporter cells ( A ) and Caco-2-AhR reporter cells ( B ) were incubated with AhR ligand antagonists (CH-223191 1 µM and GNF-351 1 µM) and an HSP90 inhibitor ((−)-Epigallocatechin-3-gallate, EGCG, 20 µM) prior stimulation with butyrate (But, 2 mM) and TCDD (10 nM) for 24 h. ( C ) Relative expression of CYP1A1 on HT-29 cells treated during 6 h with 2 mM butyrate or TCDD 10 nM in presence or absence of AhR antagonists. CYP1A1 expression is normalised to control expression (unstimulated cells) and is determined by the 2 −ΔΔCt method using β-actin as control gene. ( D ) Relative expression of CYP1A1 on HT-29 cells transfected with control siRNA (white bars) or AhR siRNA (grey bars) and incubated during 6 h with 2 mM butyrate or TCDD 10 nM. CYP1A1 expression is normalised to stimulated control expression (cells transfected with control siRNA and stimulated with either butyrate or TCDD), expressed in percentage and is determined by the 2 −ΔΔCt method using β-actin as control gene. Data are means ± SEM of at least three distinct experiments, performed in triplicate. ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001.

Journal: Scientific Reports

Article Title: Identification of the novel role of butyrate as AhR ligand in human intestinal epithelial cells

doi: 10.1038/s41598-018-37019-2

Figure Lengend Snippet: AhR antagonists inhibit AhR activation by butyrate. HT-29-AhR reporter cells ( A ) and Caco-2-AhR reporter cells ( B ) were incubated with AhR ligand antagonists (CH-223191 1 µM and GNF-351 1 µM) and an HSP90 inhibitor ((−)-Epigallocatechin-3-gallate, EGCG, 20 µM) prior stimulation with butyrate (But, 2 mM) and TCDD (10 nM) for 24 h. ( C ) Relative expression of CYP1A1 on HT-29 cells treated during 6 h with 2 mM butyrate or TCDD 10 nM in presence or absence of AhR antagonists. CYP1A1 expression is normalised to control expression (unstimulated cells) and is determined by the 2 −ΔΔCt method using β-actin as control gene. ( D ) Relative expression of CYP1A1 on HT-29 cells transfected with control siRNA (white bars) or AhR siRNA (grey bars) and incubated during 6 h with 2 mM butyrate or TCDD 10 nM. CYP1A1 expression is normalised to stimulated control expression (cells transfected with control siRNA and stimulated with either butyrate or TCDD), expressed in percentage and is determined by the 2 −ΔΔCt method using β-actin as control gene. Data are means ± SEM of at least three distinct experiments, performed in triplicate. ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001.

Article Snippet: AhR antagonists: CH-22319 (1 µM, Millipore/Calbiochem), GNF-351 (1 µM, Millipore/Calbiochem), (−)Epigallocathechin gallate (20 µM, EGCG, Sigma).

Techniques: Activation Assay, Incubation, Expressing, Transfection